Product Name |
Firefly Luciferase mRNA, modified with Cy5
|
Description |
This Cy5 modified firefly luciferase mRNA can be visualized under fluorescence microscope using a Cy5 filter for the analysis of delivery efficiency. The expression efficiency can be quantified by luciferase assay |
Modifications |
- 5' Capped with ARCA (Anti Reverse Cap Analog,
m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG
(m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
- Cyanine Cy5-UTP:N1-Methyl-Pseudouridine-5'-Triphosphate at a ratio of 1:3
- PolyA: 180 nt
|
Application |
Report assay |
Concentration |
~1 µg/µl |
Transfection Protocol |
Lipofectamine™
MessengerMAX™ Transfection Reagent Protocol |
QC |
Agarose gel electrophoresis |
Shipping |
Dry ice |
Storage |
-80 oC |