| Product Name |
Firefly Luciferase mRNA, modified with
Cy3
|
| Description |
This Cy3 modified firefly luciferase
mRNA can be visualized under fluorescence microscope using a Cy3 filter
for the analysis of delivery efficiency. The expression efficiency can
be quantified by luciferase assay |
| Modifications |
- 5' Capped with ARCA (Anti Reverse Cap Analog,
m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG
(m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
- Cyanine Cy3-UTP:N1-Methyl-Pseudouridine-5'-Triphosphate at
a ratio of 1:3
- 5mCTP
- PolyA: 180 nt
|
| Application |
Report assay |
| Concentration |
~1 µg/µl |
| Transfection Protocol |
Lipofectamine™
MessengerMAX™ Transfection Reagent Protocol |
| QC |
Agarose gel electrophoresis |
| Shipping |
Dry ice |
| Storage |
-80 oC |