| Product Name |
NLS-FLP Recombinase mRNA,
modified with Cyanine 5-UTP (Cy5) and Pseudo-UTP at 1:3 ratio |
| Description |
This NLS-FLP mRNA will express human codon optimized Flp
recombinase fused to nuclear localization sequence. The mRNA can be
used for FRT recombination in cell lines and primary cells. The
expression of NLS-FLP protein can be visualized by a Cy5 filter.
|
| Codon Optimization |
Human
|
| Modifications |
- 5' Capped with ARCA (Anti Reverse Cap Analog,
m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG
(m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
- Cyanine Cy5-UTP:N1-Methyl-Pseudouridine-5'-Triphosphate at
a ratio of 1:3
- 5mCTP
- PolyA: 180 nt
|
| Application |
ex vivo FRT recombination |
| Concentration |
~1 µg/µl |
| Transfection Protocol |
Lipofectamine™
MessengerMAX™ Transfection Reagent Protocol |
| QC |
- Agarose gel electrophoresis
- Activity assay: HEK293 cells transfected with FRT reporter
plasmid
|
| Shipping |
Dry ice |
| Storage |
-80 oC |