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FLP recombinase catalyzes the
site-specific recombination of DNA
between FRT sites. It has been widely used for generating conditional
knockout mice, mediated by FLP/FRT system. Codon optimized FLP is a
human/mouse
codon-optimized thermo-stable mutant form of
FLP, with enhanced recombinase activity by 4-10 fold higher than FLP at
37°C.
The self-amplifying NLS-Flp mRNA (SA-NLS-Flp) encodes four nonstructural proteins (NSP1 to NSP4) derived from a positive-stranded alphavirus venezuelan equine encephalitis virus (VEEV), in addition to GOI NLS-Flp. Upon entering the cytoplasm, the mRNA is translated to produce replicase complex, which amplifies the original NLS-Flp mRNA template to produce numerous copies of itself (mRNA replication), thus driving robust NLS-Flp protein expression.
SA-NLS-Flp mRNA induces much higher ex vivo recombination efficiency in cells than NLS-Flp mRNA with lower dose of delivery.
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