Human Klf4-Oct4-Sox2-Glis1-c-Myc (KOSGM) Self-Amplifying mRNA

Cat #: EG-312

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Product Name	Human Klf4-Oct4-Sox2-Glis1-c-Myc (KOSGM) Self-Amplifying mRNA
Description	This self-amplifying mRNA encodes four nonstructural proteins (nsP1 to nsP4) derived from a positive-stranded alphavirus venezuelan equine encephalitis virus (VEEV), and five human transcription factors (OCT4, KLF4, c-MYC, SOX2, GLIS1) linked by four 2A self-cleaving peptides
Applications	Efficient, rapid, integration-free generation of pluripotent stem (iPS) cells from human fibroblasts.
Modifications	5' Capped with vaccinia capping enzyme system and 2'-O-methyltransferase to yield cap-1 structure Pseudo-UTP 5mCTP PolyA: 180 nt
Concentration	~1 µg/µl
Transfection Protocol	Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC	Agarose gel electrophoresis
Shipping	Dry ice
Storage	-80 oC
References	Reprogramming human fibroblasts to pluripotency using modified mRNA. Mandal & Rossi, Nature Protocols 8,568-582 (2013) \| doi:10.1038/nprot.nprot.2013.019.