| Description |
Bsu DNA Polymerase I, Large Fragment catalyzes 5´→ 3´ synthesis of DNA lacks 5´→ 3´ and 3´→ 5´ exonuclease activity.
|
| Applications |
- Random primer labeling
- Second strand cDNA synthesis
- Single dA tailing
- Strand displacement DNA synthesis
- Recombinase polymerase amplification
|
| Source |
A recombinant E. coli strain carrying the large fragment of Bacillus subtilis DNA polymerase I gene |
| Unit Definition |
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C. |
| Components |
- Bsu DNA Polymerase: 5,000 U/ml in 10 mM Tris-HCl, 50 mM KCl, 1.0 mM Dithiothreitol, 0.1 mM EDTA, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25°C
- 10X Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl2, 10 mM DTT pH 7.9 @ 25°C
|
| Quality Control |
The absence of endonuclease, nicking activity and exonuclease activity is confirmed by appropriate quality tests. |
| Storage Condition |
-20 °C
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