| Description |
Poly(A) Polymerase uses ATP as a substrate for template-independent addition of AMP from ATP to the 3´ hydroxyl termini of RNA molecules.
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| Applications |
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Addition of a poly(A) tail to RNA synthesized in vitro for increasing RNA stability and translation efficiency prior to transferring into eukaryotic cells
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cDNA library construction using a primer containing poly(dT) on its 3´ end.
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Synthesis of polyadenylated RNA for nucleic acid amplification
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| Source |
A recombinant E. coli strain carrying E. coli Poly(A) Polymerase gene |
| Unit Definition |
1 unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-insoluble material in 10 minutes at 37°C. |
| Components |
- Poly(A) Polymerase: 5,000 U/ml in 20 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 1 mM EDTA, 50% Glycerol, 0.1% Triton® X-100, pH 7.5 @ 25°C
- 10X Reaction Buffer: 500 mM Tris-HCl, 2.5 M NaCl, 100 mM MgCl2, pH 7.9 @ 25°C
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| Quality Control |
The absence of endonuclease, nicking activity, exonuclease and RNAse activity is confirmed by appropriate quality tests. |
| Storage Condition |
-20 °C
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