| Description |
Recombinant High Fidelity 3C Thermostable DNA Polymerase (3C HiFi) is purified from E. coli transformed with an engineered DNA polymerase gene similar to a thermostable DNA polymerase from Thermococcus. 3C HiFi can amplify target DNA up to 20 kb. The processivity and elongation rate of 3C HiFi are at least 5 times higher than that of Pfu DNA polymerase. |
| Application |
- High fidelity PCR amplification
- Blunt-end PCR cloning
|
| Source |
A recombinant E. coli strain carrying an engineered thermostable DNA polymerase |
| Unit Definition |
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material for 30 min at 72°C. |
| Components |
- 3C HiFi Polymerase: 5 units in storage buffer, 20 mM Tris-HCl (pH 8.0), 40 mM NaCl, 2 mM Sodium Phosphate, 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% (v/v) glycerol
- 10X Reaction Buffer.
|
| Storage Condition |
-20 °C
|