| Description |
DNA Polymerase I (E. coli) is a mesophilic DNA polymerase that exhibits 5′-3′ DNA synthesis in addition to both 3′→ 5′ and 5′→ 3′ exonuclease activities. The combination of DNA synthesis and 5′→ 3′ nuclease characteristics enable nick-translation during DNA synthesis. |
| Applications |
- Nick translation of DNA in conjunction with DNase to obtain probes with a high specific activity
- Second strand synthesis of cDNA in conjunction with RNase H
|
| Source |
A recombinant E. coli strain carrying E coli PolA gene. |
| Unit Definition |
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 37°C. |
| Components |
- DNA Polymerase l: 10,000 U/ml in 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C
- 10X Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl2, 10 mM DTT, pH 7.9 @ 25°C
|
| Quality Control |
The absence of endodeoxyribonucleases confirmed by appropriate quality test. |
| Storage Condition |
-20 °C |