| Product Name |
T7 DNA Polymerase |
| Catalog Number |
EG-1059 |
| Description |
T7 DNA Polymerase, a highly processive enzyme composed of an 80 kDa T7 bacteriophage gene 5 protein and a 12 kDa Escherichia coli thioredoxin (trxA gene product) expressed recombinantly in Escherichia coli, catalyzes 5’ to 3’ DNA synthesis with robust 3’ to 5’ exonuclease activity on single- and double-stranded DNA, ideal for long-template synthesis and DNA labeling. |
| Applications |
- Purification of covalently closed circular DNA by removing residual genomic DNA
- Primer extension reactions on long templates
- DNA 3’-end labeling
- Strand extension in site-directed mutagenesis
- Fill-in blunting of 5’ overhangs
- Second-strand cDNA synthesis
- In situ detection of DNA fragmentation associated with apoptosis
|
| Source |
Recombinant Escherichia coli co-expressing the T7 bacteriophage gene 5 and Escherichia coli trxA gene |
| Unit Definition |
One unit is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard DNA polymerase assay conditions. |
| Components |
- T7 DNA Polymerase: 10,000 units/mL in 50 mM potassium phosphate (pH 7.0 at 25°C), 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol
- 10X Reaction Buffer: 200 mM Tris-HCl (pH 7.5 at 25°C), 100 mM MgCl2, 10 mM DTT
|
| Quality Control |
- Absence of endodeoxyribonucleases confirmed by appropriate quality tests
- Functionally validated for primer extension on long templates
|
| Storage |
-20°C |
| Shipping |
Dry ice |