Description |
Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of single or double stranded DNA molecules. The presence of 1 mM Co2+ stimulates the tailing of the 3'-ends of DNA fragments.
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Applications |
- Addition of homopolymer tails to the 3' ends of DNA
- Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP)
- TUNEL assay (in situ localization of apoptosis)
- TdT dependent PCR
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Source |
A recombinant E. coli strain carrying an engineered human terminal deoxynucleotidyl transferase gene |
Unit Definition |
1 unit is defined as the amount of polymerase required to convert 1 nmol of dATPs into acid insoluble material in 1 hour at 37°C.
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Components |
- Terminal deoxynucleotidyl Transferase: 20,000 U/ml in 50 mM KPO4, 100 mM NaCl, 1.0 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% Triton X-100, pH 7.3 @ 25°C
- 10X Reaction Buffer: 200 mM Tris-Acetate, 500 mM Potassium Acetate, 100 mM Magnesium Acetate, 10 mM DTT, pH 7.9 @ 25°C
- 2.5 mM CoCl2
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Storage Condition |
-20 °C |