Recombinant Cre recombinase (TAT-Cre) was purified from an HEK293 cell line expressing enhanced form of Cre Recombinase from bacteriophage P1. This Cre recombinase has an N-terminal 6XHis tag, a Tat peptide (GRKKRRQRRRPPAGTSVSL) and an NLS sequence (PKKKRKV). Tat-Cre is the most effective protein in transduction (in vivo) and subsequent recombination compared to other forms of Cre recombinases, e.g., HNC, TCH6, HC, HNCM, CH.

• In vitro LoxP recombination for subcloning or vector/clone engineering

• Transduction into cultured cells including stem cells ex vivo

QC: HEK293T- Cre reporter cell line 80~100% recombination efficiencies.

Figure 1. QC assay of Tat-Cre. Adding  50 and 100 units of Tat-Cre to HEK 293T cells transfected with LoxP-RFP-Stop-LoxP-GFP reporter plasmid DNA.

Endotoxin Levels: < 0.1 EU/ug