The self-amplifying NLS-Cre mRNA (SA-NLS-Cre) encodes four nonstructural proteins (NSP1 to NSP4) derived from a positive-stranded alphavirus venezuelan equine encephalitis virus (VEEV), and human codon-optimized Cre recombinase fused to nuclear localization sequence. Upon entering the cytoplasm, the mRNA is translated to produce replicase complex, which amplifies the original NLS-Cre mRNA template to produce numerous copies of itself (mRNA replication), thus driving robust NLS-Cre protein expression. It can be used for loxP recombination in cell lines and primary cells

• 5' Capped with vaccinia capping enzyme system and 2'-O-methyltransferase to yield cap-1 structure
• Pseudo-UTP
• 5mCTP
• PolyA: 180 nt

• Agarose gel electrophoresis
• Activity assay: HEK293 cells transfected with LoxP reporter plasmid