| Product Name |
NLS-Cre Recombinase (NLS-Cre) mRNA,
modified with Cyanine 5-UTP (Cy5) and Pseudo-UTP at 1:3 ratio |
| Description |
This NLS-Cre mRNA will express human/mouse codon optimized Cre
recombinase fused to nuclear localization sequence. The mRNA can be
used for loxP recombination in cell lines and primary cells. The
expression of NLS-Cre protein can be visualized by a Cy5 filter.
|
| Codon Optimization |
Human & mouse |
| Modifications |
- 5' Capped with ARCA (Anti Reverse Cap Analog,
m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG
(m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
- Cyanine Cy5-UTP:N1-Methyl-Pseudouridine-5'-Triphosphate at
a ratio of 1:3
- 5mCTP
- PolyA: 180 nt
|
| Application |
ex vivo LoxP recombination |
| Concentration |
~1 µg/µl |
| Transfection Protocol |
Lipofectamine™
MessengerMAX™ Transfection Reagent Protocol |
| QC |
- Agarose gel electrophoresis
- Activity assay: HEK293 cells transfected with LoxP reporter
plasmid
|
| Shipping |
Dry ice |
| Storage |
-80 oC |