| Product Name |
Thermus thermophilus MutS DNA mismatch repair protein |
| Size |
100 µg |
| Description |
The Tth MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases. This 91.8-kDa protein binds in vitro to heteroduplex DNAs containing mispaired or unpaired bases over a wide temperature range from 4 to 70 °C and has a thermostable ATPase activity. This thermostable Tth MutS is active at temperature between 0 to 75°C. Since Tth MutS efficiently binds to 1-4 bases deletion (or insertion) and mismatch base pairs of GT, CT and AG, it is useful for detecting these mutations. Mutations can be detected in polyacrylamide gels or on a solid phase such as Ni agarose or magnetic Ni-NTA particles.
|
| Applications |
- Remove mismatch DNA (error correction) from gene synthesis reaction
- Mutation detection and removal
- Rapid isothermal SNP detection
|
| Source |
E. coli |
| Fusion Tag |
6XHis tag at N-terminus |
| Purification Method |
FPLC |
| Purity |
~ 95% as determined by SDS-PAGE
|
| Accession # |
WP_011173381 |
| Gene Name |
Thermus thermophilus MutS DNA mismatch repair protein
|
| MW |
135.9 kDa |
| Protein Sequence |
1 MGSSHHHHHH MGNMLKGEGP GPLPPLLQQY VELRDRYPDY LLLFQVGDFY ECFGEDAERL
61 ARALGLVLTH KTSKDFTTPM AGIPIRAFDA YAERLLKMGF RLAVADQVEP AEEAEGLVRR
121 EVTQLLTPGT LTQEALLPRE ANYLAAIATG DGWGLAFLDV STGEFKGTLL KSKSALYDEL
181 FRHRPAEVLL APELRENEAF VAEFRKRFPV MLSEAPFEPQ GEGPLALRRA QGALLAYARA
241 TQGGALSVRP FRLYDPGAFV RLPEASLKAL EVFEPLRGQD TLFGVLDETR TAPGRRLLQA
301 WLRHPLLERG PLEARLDRVE RFVREGALRE GVRRLLFRLA DLERLATRLE LSRASPRDLA
361 ALRRSLEILP ELKGLLGEEV GLPDLSGLLE ELRAALVEDP PLKVSEGGLI REGYDPDLDA
421 LRRAHAEGVA YFLDLEAREK ERTGIPTLKV GYNAVFGYYL EVTRPYYEKV PQEYRPVQTL
481 KDRQRYTLPE MKERERELYR LEALIKRREE EVFLALRERA RKEAEALREA ARILAELDVY
541 AALAEVAVRH GYTRPRFGER LRIRAGRHPV VERRTAFVPN DLEMAHELVL VTGPNMAGKS
601 TFLRQTALIA LLAQIGSFVP AEEAELPLFD GIYTRIGASD DLAGGKSTFM VEMEEVALVL
661 KEATERSLVL LDEVGRGTSS LDGVAIATAL AEALHERRCY TLFATHYFEL TALALPRLKN
721 LHVAAKEEEG GLVFYHQVLP GPASKSYGVE VAEMAGLPKE VVERARALLS AMAARREGAL
781 EEVLERLLAL DPDRLTPLEA LRFLHELKAL ALGLPLGSMK G |
| Storage Buffer |
20 mM Hepes, pH 7.4, 250 mM NaCl, 0.1mM EDTA, 1 mM DTT, 50% Glycerol |
| Reaction Buffer |
100 mM KCl, 50mM Tris-HCl, pH 8.5, 5~20 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 2% Glycerol, 65 °C |
| Storage |
-20 to -80 °C. |
| Shipping |
4°C or dry ice |
| References |
1. Protein-mediated error correction for de novo DNA synthesis. Nucleic Acids Res. 2004 Nov 23;32(20):e162.
2. Correcting errors in synthetic DNA through consensus shuffling. Nucleic Acids Res. 2005 Mar 30;33(6):e55.
3. MutS as a tool for mutation detection. Acta Biochim Pol. 2005;52(3):575-83. Epub 2005 Aug 4.
4. One tube mutation detection using sensitive fluorescent dyeing of MutS protected DNA. Nucleic Acids Res. 2000 Apr 15;28(8):E36.
5. Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology. Nature Methods - 4, 257 - 262 (2007) doi:10.1038/nmeth1007
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