| Product Name |
Anti-B7-H6 monoclonal
antibody, recombinant |
| Cat # |
EG-370 |
| Size |
100 µg |
| Key features |
Rabbit monoclonal antibody
produced in 293 cells with serum-free, protein-free medium, purified
with protein A column;
High batch-to-batch consistency and reproducibility;
Long term security of supply; |
| Clone number |
M18 |
| Immunogen |
Recombinant extracellular
domain of B7-H6 that was expressed and purified from 293 cells |
| Reaction
target |
human B7-H6 |
| Species
Cross-Reactivity |
Human |
| Applications |
Flow Cytometry; IP; ELISA; |
| Working
concentrations |
Flow cytometry and ELISA: 5
µg/ml of final concentration is suggested.
IP: 10 µg in 0.3-0.5 mg cell lysates is suggested.
|
| Background |
B7 homolog 6 (B7-H6), a
novel member of the B7 family, was identified on tumor cell surfaces.
B7-H6 promotes tumorigenesis via apoptosis inhibition, proliferation
and immunological progression. B7-H6 might be a valuable biomarker and
target for
immunotherapy, diagnostics, and prognostics in cancer. |
| QC |
Flow cytometry assay showing cell binding intensity. 1
ml of ZR5 cells (10^6) were incubated with 5 µg B7-H6 monoclonal
antibody, and detected with Cy5-conjugated goat-anti-rabbit antibody.
Shaded area, isotype control antibody, cyan line, B7-H6 antibody
staining.
|
| Concentration |
As indicated on the vials |
| Formulation |
80% PBS, pH 7.4; 20%
Glycerol; 5% Trehalose; 0.04% Sodium azide as Preservative |
| Shipping |
Blue ice |
| Storage |
-20 to -70 oC
(long term)
or 4 oC (one week).
Depending on the expected frequency of use, the product can be
aliquoted to avoid heavily repeated freezing/thawing cycles. |