| Product Name |
Anti-Mesothelin monoclonal
antibody, recombinant |
| Cat # |
EG-375 |
| Size |
100 µg |
| Key features |
Rabbit monoclonal antibody
produced in 293 cells with serum-free, protein-free medium, purified
with protein A column;
High batch-to-batch consistency and reproducibility;
Long term security of supply; |
| Clone number |
R47 |
| Immunogen |
Recombinant N-terminal
mesothelin fragment (residues 296–390) that was expressed and purified
from 293 cells |
| Reaction
target |
N-terminal region of
mesothelin |
| Species
Cross-Reactivity |
Human |
| Applications |
Flow Cytometry; IP;
ELISA;Western blot |
| Working
concentrations |
Flow cytometry and ELISA: 5
µg/ml of final concentration is suggested.
IP: 10 µg in 0.3-0.5 mg cell lysates is suggested.
Western blot: 1 µg/ml.
|
| Background |
Mesothelin gene encodes a
71-kDa precursor protein that is processed to a 40-kDa protein termed
mesothelin, which is a glycosylphosphatidylinositol (GPI)-anchored
glycoprotein present on the cell surface. Mesothelin is a
differentiation antigen that is present on a restricted set of normal
adult tissues such as the mesothelium. In contrast, it is overexpressed
in a variety of cancers including ovarian cancers, non-small cell lung
cancer (NSCLC), colorectal cancer (CRC) and mesothelioma. Its limited
distribution on normal tissues, relatively specific expression at high
levels in tumors and important role in tumor development and prognosis,
make mesothelin a potential target for tumor immunotherapy. |
| QC |
Flow cytometry assay using R47 monoclonal antibody.
A431 is mesothelin negative cells. A431 (mesothelin++) is derived from
A431 that was forcefully over-expressing mesothelin. One million cells
were incubated with 1 µg antibody in 1 ml PBS buffer containing 5% BSA.
Antibody binding was detected with Cy5-conjugated goat-anti-rabbit
antibody. Blue line, isotype control antibody staining; Red line, R47
staining.
|
| Concentration |
As indicated on the vials |
| Formulation |
80% PBS, pH 7.4; 20%
Glycerol; 5% Trehalose; 0.04% Sodium azide as Preservative |
| Shipping |
Blue ice |
| Storage |
-20 to -70 oC
(long term)
or 4 oC (one week).
Depending on the expected application frequency, the product can be
aliquoted to avoid heavily repeated freezing/thawing cycles. |