| Product Name |
Anti-SIRPa monoclonal
antibody, recombinant |
| Cat # |
EG-376 |
| Size |
100 µg |
| Key features |
Rabbit monoclonal antibody
produced in 293 cells with serum-free, protein-free medium, purified
with protein A column;
High batch-to-batch consistency and reproducibility;
Long term security of supply; |
| Clone number |
M181 |
| Immunogen |
Recombinant extracellular
domain of SIRPa that was expressed and purified from 293 cells |
| Reaction
target |
Extracellular region of
SIRPa |
| Species
Cross-Reactivity |
Human |
| Applications |
Flow Cytometry; IP;
ELISA; |
| Working
concentrations |
Flow cytometry and ELISA: 5
µg/ml of final concentration is suggested.
IP: 10 µg in 0.3-0.5 mg cell lysates is suggested.
|
| Background |
SIRPa (CD47-signal
regulatory protein alpha) is the receptor of CD47 (a "don't eat me"
signal for phagocytic cells that is commonly overexpressed on the
surface of solid tumor cells). SIRPa is preferentially expressed on
Macrophage and dentritic cells, serving as immune checkpoint inhibitory
molecule of these cells. The binding of aberrantly expressed CD47 on
tumor cells to SIRPa on macrophages allows tumor cells to evade immune
destruction. Blockade of SIRPa-CD47 interaction with monoclonal
antibodies enabled macrophage phagocytosis of tumor cells that were
otherwise protected (1). Therefore SIRPa is an attractive target for
immunotherapy of cancers.
References:
Stephen B Willingham, et al. The CD47-signal regulatory protein alpha
(SIRPa) interaction is a therapeutic target for human solid tumors.
PNAS. 2012;109:6662-7
. |
| QC |
Flow cytometry analysis using M181 monoclonal antibody.
A431 is SIRPa negative cells. A431 (SIRPa++) is derived from A431 that
was forcefully over-expressing SIRPa. One million cells were incubated
with 5 µg antibody in 1 ml PBS buffer containing 5% BSA. Antibody
binding was detected with Cy5-conjugated goat-anti-rabbit antibody.
Shade area, isotype control antibody staining; Red line, M181 staining.
|
| Concentration |
As indicated on the vials |
| Formulation |
80% PBS, pH 7.4; 20%
Glycerol; 5% Trehalose; 0.04% Sodium azide as Preservative |
| Shipping |
Blue ice |
| Storage |
-20 to -70 oC
(long term)
or 4 oC (one week).
Depending on the expected application frequency, the product can be
aliquoted to avoid heavily repeated freezing/thawing cycles. |