| Cat # |
EG-323 |
| Description |
A recombinant form of South African variant of the spike glycoprotein receptor binding domain (RBD, Arg319-Phe541) from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2). This RBD mutant has K417N, E484K and N501Y mutations in the S glycoprotein RBD as compared to the SARS-CoV-2 reference sequence (GenPept: QHD43416). It was produced in HEK293 cells and purified by nickel affinity and ion exchange chromatography. This recombinant protein contains 223 residues of the SARS-CoV-2 spike glycoprotein RBD with a C-terminal hexa-histidine tag. |
| Mutations |
K417N, E484K and N501Y |
| Tags |
C-terminal hexa-histidine |
| Purification |
FPLC by nickel affinity and ion exchange chromatography |
| Sequence |
1 RVQPTESIVR FPNITNLCPF GEVFNATRFA SVYAWNRKRI SNCVADYSVL
51 YNSASFSTFK CYGVSPTKLN DLCFTNVYAD SFVIRGDEVR QIAPGQTGNI
101 ADYNYKLPDD FTGCVIAWNS NNLDSKVGGN YNYLYRLFRK SNLKPFERDI
151 STEIYQAGST PCNGVKGFNC YFPLQSYGFQ PTYGVGYQPY RVVVLSFELL
201 HAPATVCGPK KSTNLVKNKC VNFHHHHHH
|
| Mol Wt |
25.96 kDa |
| Source |
HEK 293 |
| Purity |
90~95% by SDS-PAGE |
| Applications |
Western blot, ELISA and animal vaccination
|
| Storage Buffer |
PBS + 50% Glycerol |
| Storage |
-20ºC ~ -80ºC |
| Shipping |
dry ice |
| References |
|