Product Name |
Vesicular Stomatitis Virus (VSV) Pseudotyped with SARS-CoV-2
Spike Glycoprotein, HexaPro Stabilized Trimer
|
Description |
Vesicular stomatitis virus particles
pseudotyped with SARS-CoV-2
spike glycoprotein, with hexaPro mutations so the surface protein
antigenically optimal pre-fusion conformation, produced and purified
from HEK 293 cells.
|
Mutations |
Furin site RRAR mutated to GSAS; HexaPro
mutations (K986P, V987P, F817P, A892P, A899P and A942P) to ensure the S
glycoprotein remains in an antigenically optimal pre-fusion
conformation
|
Source
|
HEK293T cells
|
Reporter
|
EGFP
|
Application |
- Screening of SARS-CoV-2
neutralizing antibodies
-
Assessment of vaccine efficacy
|
Titer |
> 1 X 107 RFU/ml or > 1
X 105 TU/ml
|
Shipping |
Dry ice |
Storage |
-80 oC |
References
|
Generation of VSV Pseudotypes Using Recombinant ΔG-VSV for Studies on Virus Entry, Identification of Entry Inhibitors, and Immune Responses to Vaccines Sci Rep 10, 19076 (2020). doi:10.1016%2Fj.jviromet.2010.08.006
|