| Product Name |
Vesicular Stomatitis Virus (VSV) Pseudotyped with SARS-CoV-2 Spike Glycoprotein, HexaPro Stabilized Trimer, United Kingdom Variant |
| Description |
Vesicular stomatitis virus particles pseudotyped with SARS-CoV-2 spike glycoprotein, with hexaPro mutations so the surface protein antigenically optimal pre-fusion conformation, produced and purified from HEK 293 cells. This
pseudovirus has N501Y mutation. |
| Mutations |
Furin site RRAR mutated to GSAS; HexaPro mutations (K986P, V987P, F817P, A892P, A899P and A942P) to ensure the S glycoprotein remains in an antigenically optimal pre-fusion conformation.
United Kingdom mutations: N501Y
|
| Source |
HEK293T cells |
| Reporter |
EGFP |
| Application |
- Screening of SARS-CoV-2 neutralizing antibodies
- Assessment of vaccine efficacy
|
| Titer |
> 1 X 107 RFU/ml or > 1 X 105 TU/ml |
| Shipping |
Dry ice |
| Storage |
-80 oC |
| References |
Generation of VSV Pseudotypes Using Recombinant ΔG-VSV for Studies on Virus Entry, Identification of Entry Inhibitors, and Immune Responses to Vaccines Sci Rep 10, 19076 (2020). doi:10.1016%2Fj.jviromet.2010.08.006 |