| Description |
E. coli Uracil-DNA Glycosylase (UDG) catalyses the release of uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from short oligonucleotides (<6 bases), or RNA substrates.
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| Applications |
- Digestion of uracil-containing DNA
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| Source |
An E. coli strain that carries the ung (Uracil-DNA Glycosylase) gene from E. coli K-12. |
| Unit Definition |
One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA. |
| Components |
- E coli UDG: 5,000 units/ml in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C
- 10X Reaction Buffer: 200mM Tris-HCI, 10 mM DTT, 10 mM EDTA, pH 8.0 @ 25°C
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| Quality Control |
The absence of single and double-strand exonuclease, and endodeoxyribonucleases is confirmed by appropriate quality tests
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| Storage Condition |
-20 °C
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