| Description |
Inorganic Pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphates. The enzyme requires a divalent metal cation with Mg2+ for activity |
| Applications |
- High yield RNA synthesis by in vitro transcription
- DNA polymerization reactions: preventing accumulation of pyrophosphate
- Removal of contaminant PPi in reagents used for SNP genotyping by methods based on the detection of pyrophosphate
|
| Source |
Purified from a recombinant E. coli strain carrying the E coli inorganic pyrophosphatase (ppa) gene |
| Components |
- Inorganic Pyrophosphatase, 100 U/ml in storage buffer: 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 8.0 @ 25°C
- 10 X Reaction Buffer: 200 mM Tris-HCl, 20 mM MgCl2, pH 7.2 @ 25°C
|
| Unit Definition |
One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 20 mM Tris-HCl, pH 7.2, 2 mM MgCl2 and 2 mM PPi). |
| Quality Control |
The absence of endo-, exodeoxyribonucleases, phosphatases, and ribonucleases is confirmed by appropriate quality tests.
|
| Storage |
-20°C. |