Recombinant adenoviruses will be produced by homologous recombination in 293A cells.

Steps

• Starting with a recombinant adenovirus transfer vector, E. coli or AdExpress-293A cells will be transformed to yield recombinant adenoviral DNA.
• Generation of recombinant by transfecting AdExpress-293A cells with recombinant viral DNA.
• Plaque purification and identification of the recombinant adenovirus by PCR or Western blot. If no amplicifcation is requested, eluted plaques are delivered to the client.

Time Required

AdExpress: 2 to 3 weeks

AdEasy: 4 to 8 weeks