| Cat # |
EG-329
|
| Description |
A recombinant form of the nucleocapsid (N) protein from severe acute respiratory syndrome-related
coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced
in HEK293 cells and purified by nickel affinity and ion exchange
chromatography.
|
Mutations
|
WT |
Tags
|
C-terminal hexa-histidine |
Purification
|
FPLC by nickel
affinity and ion exchange chromatography |
| Sequence |
1 MSDNGPQNQR NAPRITFGGP SDSTGSNQNG ERSGARSKQR RPQGLPNNTA
51 SWFTALTQHG KEDLKFPRGQ GVPINTNSSP DDQIGYYRRA TRRIRGGDGK
101 MKDLSPRWYF YYLGTGPEAG LPYGANKDGI IWVATEGALN TPKDHIGTRN
151 PANNAAIVLQ LPQGTTLPKG FYAEGSRGGS QASSRSSSRS RNSSRNSTPG
201 SSRGTSPARM AGNGGDAALA LLLLDRLNQL ESKMSGKGQQ QQGQTVTKKS
251 AAEASKKPRQ KRTATKAYNV TQAFGRRGPE QTQGNFGDQE LIRQGTDYKH
301 WPQIAQFAPS ASAFFGMSRI GMEVTPSGTW LTYTAAIKLD DKDPNFKDQV
351 ILLNKHIDAY KTFPPTEPKK DKKKKADETQ ALPQRQKKQQ TVTLLPAADL
401 DDFSKQLQQS MSSADSTQAH HHHHH
|
Mol Wt
|
46.5 kDa |
Source
|
HEK 293 |
Purity
|
90~95% by SDS-PAGE |
|
Applications
|
ELISA, Western Blot, Lateral Flow Assay
|
| Storage Buffer |
PBS + 50% Glycerol
|
| Storage |
-20ºC ~ -80ºC |
| Shipping |
dry ice |
References
|
|