| Service Name |
Transient Protein Production using HEK 293 Suspension Cells |
| Description |
Our Transient Protein Production service utilizes high-efficiency, suspension-cultured HEK 293 cells (293F or 293E) for rapid, scalable expression of recombinant proteins. This platform is ideal for generating native, active, and high-purity proteins—including Fc fusion proteins and other tagged constructs—for functional, therapeutic, and structural studies. Our optimized protocols and advanced AKTA FPLC purification systems enable production of up to 1 gram of protein in as little as 4–14 days, supporting applications from assay development to preclinical research.
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| Key Features & Advantages |
- Transient expression in suspension-adapted HEK 293F or 293E cells
- Rapid production: up to 1 g protein in 4–14 days
- Native folding and post-translational modifications
- High purity (>95%) protein via AKTA FPLC purification
- Fc fusion proteins with removable tags (e.g., cleavable by 3C protease)
- Serum-free, scalable culture conditions
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| Workflow & Timeline |
- Cloning and Subcloning (2 weeks):
- PCR amplification and cloning into mammalian expression vector
- Sequence verification and plasmid preparation
- Expression Optimization (2 weeks):
- Transient transfection of HEK 293 suspension cells
- Assessment of expression by SDS-PAGE, dot-blot, or Western blot
- Large-Scale Expression & Purification (2 weeks):
- Transfection of up to 1 L HEK 293 cells in serum-free medium
- Protein purification using AKTA FPLC (affinity, IEX, SEC as needed)
- Dialysis, concentration, and QC (SDS-PAGE, Western blot, protein quantitation)
- Delivery of purified, ready-to-use protein
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| Deliverables |
- Up to 1 g purified recombinant protein (>95% purity), Fc fusion protein cleavable by 3C protease if applicable
- Quality control data (SDS-PAGE, Western blot, yield)
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| Required Materials |
- Plasmid containing gene of interest and complete vector sequence (electronic format)
- or protein sequence for gene synthesis (additional fee applies)
- or accession number for cDNA sourcing
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| Applications |
- Functional and structural studies
- Therapeutic protein and antibody production
- Assay development and screening
- Preclinical research
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| Consultation & Support |
Free project consultation and technical support throughout your project.
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| Quotation & Ordering |
- Request a quotation form from customer service: crm@excellgen.com
- Submit the completed form and PO/credit card info by email, fax, or phone
- Ship samples with a hard copy of the completed form to:
Protein Services, Excellgen, Inc. 15601 Crabbs Branch Way, Rockville, MD 20855-2743
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