LvExpress^TM Lentivirus Production and Purification

Advantages:

• Fast Cloning: Transfer GOI from Gateway donor vector to lentivirus vector using Gateway LR Clonase. The latest hORFeome (v7.1) collection has 18,414 human ORFs representing at least 15,415 human genes.
• Reliable: Generate high titer lentivirus in only 5 days.
• Scalable: Lentivirus production can be scaled up to 1 or 5 L of cultured cells.
• Fast Turnaround: ~ 3 weeks are required for cloning, generation of high titer lentivirus and virus purification.
• High Titer and High Yield: Titer from 10⁷ to 10¹⁰ TU/ml. A hollow fiber tangential flow filtration (TFF) system is used to concentrate and purify lentivirus from up to 10 L culture medium (viral supernatant). This system enables us to produce high titer virus stock (10¹⁰ TU/ml) in 1 to 10 ml.
• High Purity: Lentivirus can be further purified using FPLC or ultracentrifugation.

LvExpress^TM Lentivirus Services:

1, Clone gene of interest into a lentivirus vector.
2, Large scale lentivirus production in 293 cells.
3. Lentivirus purification by ultracentrifugation and FPLC.
4, Titer determination.

Required Materials: Sequence of gene of interest or shRNA, or plasmid DNA containing gene of interest.

Turnaround Time:

• Full Service: ~ 2 to 3 weeks.
• Customized Projects: ~ 1 to 3 weeks.

Deliverable: Purified high titer (up to 1X10¹⁰ TU/ml) lentivirus.