AdExpress^TM Adenovirus Production, Amplification and Purification

AdExpress^TM Adenovirus System offers the following advantages

• Fast Cloning: Transfer GOI from Gateway donor vector to adenovirus shuttle vector using Gateway LR Clonase. The latest hORFeome (v7.1) collection has 18,414 human ORFs representing at least 15,415 human genes. This set of libary or indvidual clone can be purchased from Dana-Farber/Harvard Cancer Center or ASU's DNASU Plasmid Repository. Other human or mouse Gateway clones are available from Invitrogen (Ultimate™ ORF) or GeneCopoeia.
• Reliable: Generate high titer crude adenovirus stock in only 5 days.
• Scalable: Adenovirus production can be scaled up to 1 or 10 L of cultured cells.
• Fast Turnaround: Maximum 2 weeks are required for cloning and generation of high titer crude adenovirus stock. Another 2 weeks are needed for large scale amplification and purification.
• RCA-Free: Special cell lines can be used to produce high quality replication-competent-free adenovirus.
• High Titer: Up to 10¹³ particles/ml or 10¹² PFU/ml.
• High Purity: Adenovirus purification by CsCl ultracentrifugation and FPLC.

AdExpress^TM Adenovirus Services:

1, Clone gene of interest directly into an adenovirus vector.
2, Produce crude adenovirus stock.
3, Large scale amplification, adenovirus purification by CsCl banding (ultracentrifugation) and FPLC.
4, Titer determination.


Required Materials: Sequence of gene of interest or shRNA, or plasmid DNA containing gene of interest.

Turnaround Time:

• Full Service: ~4 to 7 weeks.
• Customized Projects: ~ 1 to 7 weeks.

Deliverable: Purified high titer (up to 1X10¹³ viral particles/ml, or 1X10¹² PFU/ml) adenovirus