| Service |
Cloning and Gene Synthesis |
| Overview |
Comprehensive molecular cloning and gene synthesis services, including subcloning, vector construction, mutagenesis, fusion protein design, and high-titer viral vector production for research applications.
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| Capabilities |
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Cloning Methods: Restriction enzyme digestion and ligation, TOPO (TA) cloning, In-Fusion cloning, Gateway cloning, and Ligation Independent Cloning (LIC).
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Viral Vector Cloning & Production: Cloning of genes into baculovirus, lentiviral, adenoviral, and adeno-associated virus (AAV) vectors. Custom production of high-titer viral particles:
- Lentivirus: 108–109 TU/mL
- Adenovirus: >1012 PFU/mL
- AAV: >1013 GC/mL
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Gene Synthesis: De novo synthesis of genes (100 bp to 5 kb) and cloning into a vector of your choice.
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Fusion Protein Construction:
- Common Expression & Purification Tags: Poly-histidine (6×His, 8×His, 9×His), MBP, GST, CBP, Thioredoxin, NusA
- Tandem Affinity Purification Tags: 6×His.GST, 6×His.MBP
- Monomeric Fluorescent Proteins: Fusion to N- or C-terminus of EGFP, BFP, CFP, YFP, RFP, IFP, IRFP, large Stokes shift RFPs
- Luciferase Fusions: Luciferase or secreted luciferase, alone or fused to fluorescent proteins via self-cleaving 2A peptides (F2A, T2A, E2A, P2A)
- Detection Tags: c-Myc, HA, Flag, 3×Flag, Halo, Strep, T7, S-Tag, SBP, V5, Xpress
- Color Tags: Soluble Red Tag (cytochrome b5, 13 kDa), Yellow Tag (FMN domain of human cytochrome P450 reductase, 21 kDa)
- Secretion Signal Peptides: For protein expression in yeast, insect, and mammalian (293/CHO) cells
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Site-Directed Mutagenesis: Single and multiple site-directed mutagenesis.
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Endotoxin-Free Plasmid Preparation: High-purity, endotoxin-free plasmid DNA suitable for sensitive applications.
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