| Product Name |
Thermus aquaticus Single-Stranded DNA-Binding Protein (TaqSSB) |
| Overview |
The single-stranded DNA-binding protein from Thermus aquaticus (TaqSSB) is a thermostable DNA-binding protein essential for DNA replication, recombination, and repair in this thermophilic bacterium. Unlike most bacterial SSBs, which are tetramers, TaqSSB forms a functional dimer, with each monomer containing two oligonucleotide/oligosaccharide-binding (OB) domains. TaqSSB binds single-stranded DNA (ssDNA) with high specificity and affinity, protecting it from nucleolytic digestion and preventing secondary structure formation, thereby facilitating efficient DNA processing at elevated temperatures[1][2][3][4][5][6][7][8].
|
| Size |
100 µg |
| Concentration |
1 mg/mL |
| Molecular Weight |
~30 kDa (monomer); functional dimer ~60 kDa[6] |
| Key Features |
- Thermostable: active and stable at high temperatures (up to 90 °C)
- Binds ssDNA with high affinity and specificity; does not bind well to double-stranded DNA[6]
- Prevents formation of secondary structures and protects ssDNA from degradation
- Unique dimeric structure, each monomer with two OB-folds[1][2][5][8]
- Flexible C-terminal region critical for protein-protein interactions with DNA polymerase III and other partners[1][2][7]
|
| Applications |
- Stabilization of ssDNA during high-temperature PCR and DNA amplification
- In vitro DNA replication, repair, and recombination assays at elevated temperatures
- Model system for studying thermostable DNA-protein interactions
|
| Source |
Recombinant protein expressed in E. coli[6] |
| Quality Control |
- Purity >95% by SDS-PAGE
- Confirmed single-stranded DNA binding activity[6]
|
| Formulation |
50 mM Tris-HCl (pH 8.0), 200 mM NaCl, 0.1 mM DTT, 0.5 mM EDTA, 50% glycerol[6] |
| Storage |
-20 °C; avoid repeated freeze-thaw cycles[6] |
| References |
- Fedorov, R., et al. 3D structure of Thermus aquaticus single-stranded DNA-binding protein gives insight into the functioning of SSB proteins. Nucleic Acids Res. 2006; 34(22):6708-6717.[1][2][5][7]
- Yoshida, M., et al. Structure of the single-stranded DNA-binding protein SSB from Thermus aquaticus. Acta Cryst. 2006; F62: 1101-1104.[8]
- UniProt Q9KH06: Thermus aquaticus SSB protein entry.[3]
- AS ONE International. Thermus aquaticus Single-stranded DNA Binding Protein (SSB) Product Sheet.[6]
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