| Product Name |
Thermus thermophilus RecA (Tth RecA), Recombinant |
| Overview |
RecA from Thermus thermophilus HB8 (Tth RecA) is a thermostable recombinase that plays a central role in homologous recombination, DNA repair, and the maintenance of genome integrity in this thermophilic bacterium. Tth RecA catalyzes ATP-dependent strand exchange between homologous DNA molecules, promotes DNA pairing, and facilitates the repair of double-stranded breaks. Its exceptional thermal stability and robust activity at elevated temperatures make it an ideal tool for in vitro recombination and strand exchange reactions under thermophilic conditions.
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| Source |
Recombinant protein expressed in E. coli carrying the Thermus thermophilus HB8 recA gene
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| Molecular Weight |
~38 kDa (monomer) |
| Key Features |
- Thermostable: active at temperatures up to 70–80 °C
- Catalyzes ATP-dependent homologous DNA strand exchange
- Promotes DNA pairing and repair of double-stranded breaks
- Facilitates in vitro recombination and genetic engineering at high temperature
- Functional homolog of E. coli RecA, but adapted for thermophilic environments
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| Applications |
- Homologous recombination and strand exchange assays at elevated temperatures
- In vitro DNA repair and recombination studies
- Thermostable genetic engineering and molecular biology workflows
- Model system for studying recombinase function in thermophiles
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| Quality Control |
- Purity >95% by SDS-PAGE
- Functional validation in ATP-dependent DNA strand exchange assays
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| Formulation |
20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5 |
| Storage |
-80 °C (long-term); avoid repeated freeze-thaw cycles |
| References |
- Yokoyama, A., et al. "Functional and structural characterization of RecA from Thermus thermophilus HB8." J Biol Chem. 2002; 277(11): 9963–9970.
- Kim, J.I., et al. "Crystal structure of RecA from Thermus thermophilus HB8 and its functional implications." Biochem Biophys Res Commun. 2002; 298(3): 385–391.
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