| Product Name |
RNase-Resistant SARS-CoV-2 mRNA qPCR Control |
| Catalog Number |
EG-368 |
| Description |
RNase-resistant SARS-CoV-2 mRNA is a stable mRNA-MS2 coat protein nanoparticle complex, produced in Escherichia coli via IPTG-induced expression of a plasmid encoding the MS2 coat protein and a SARS-CoV-2 mRNA sequence. The MS2 coat protein encapsidates the co-transcribed mRNA, forming pseudoviral-like particles that are DNA-free and resistant to RNase degradation. Stable at 4°C or room temperature for up to 6 months. |
| Sequence |
AUAAUGGACCCCAAAAUCAGCGAAAUGCACCCCGCAUUACGUUUGGUGGACCCUC 50
AGAUUCAACUGGCAGUAACCAGAAUGGAGAAGAACUGAUUACAAACAUUGGCCGC 100
AAAUUGCACAAUUUGCCCCCAGCGCUUCAGCGUUCUUCGGAAUGUCGCGCAUUGG 150
CGUUGCAACUGAGGGAGCCUUGAAUACACCAAAAGAUCACAUUGGCACCCGCAAU 200
CCUGCUAACAAUGCUGCAAUCGUGCUACAACUUCCUCAGUGUUUGCAGAUUUGGA 250
CCUGCGAGCGGGUUCUGACCUGAAGGCUCUGCGCGGACUUGUGGAGACAGCCGCU 300
CACCUUGGCUAUUGAGUGAAAUGGUCAUGUGUGGCGGUUCACUAUAUGUUAAACC 350
AGGUGGAACCUCAUCAGGAGAUGCCACAACUGCUUAUGCUAAUAGUGUUUUUAAC 400
AUUUGUCAAGCUGAAGAGACAGGUACGUUAAUAGUUAAUAGCGUACUUCUUUUUC 450
UUGCUUUCGUGGUAUUCUUGCUAGUUACACUAGCCAUCCUUACUGCGCUUCGAUU 500
GUGUGCGUACUGCUGCAAUAUUGUUAACGUGAG 533
|
| Titer |
1010 copies/µL |
| Size |
100 µL (1 × 1012 copies) |
| Format |
Liquid |
| Quality Control |
qPCR, agarose gel electrophoresis |
| Applications |
Quantitative RNA standard for RT-qPCR assays |
| Storage |
-80°C |
| Stability |
2 years at -80°C; up to 6 months at -40°C to 4°C |