NLS-eCas9 is a mutant of Streptococcus pyogenes SF370 Cas9 protein with nuclear localization signal (NLS) at N terminus. The mutant Cas9 reduces off-target effects by more than 10 folds. EGFP is linked to NLS-eCas9 via T2A peptides

• 5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
• Pseudo-UTP
• 5mCTP
• PolyA: 180 nt