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This self-amplifying mRNA encodes four
nonstructural proteins (nsP1 to nsP4) derived from a positive-stranded
alphavirus venezuelan equine encephalitis virus (VEEV), and NLS-eCas9-T2A-EGFP.
NLS-eCas9 is a mutant of Streptococcus pyogenes SF370 Cas9 protein with nuclear localization signal (NLS) at N terminus. The mutant Cas9 reduces off-target effects by more than 10 folds. EGFP is linked to NLS-eCas9 via T2A peptides
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