| Product Name |
Firefly Luciferase mRNA, Cy3-Modified |
| Overview |
This in vitro transcribed firefly luciferase mRNA incorporates Cy3-labeled nucleotides, enabling direct visualization of mRNA delivery and cellular uptake by fluorescence microscopy using a Cy3 filter set. Quantitative analysis of translation efficiency can be performed through a luciferase reporter assay.
|
| Key Features |
-
5' Cap Structure: Available with ARCA (Anti-Reverse Cap Analog; m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'-O-methyl-A)pG, Cap 1) to enhance mRNA stability and translational efficiency.
-
Nucleotide Modifications: Incorporates Cy3-UTP and N1-methyl-pseudouridine-5'-triphosphate at a 1:3 molar ratio, as well as 5-methylcytidine triphosphate (5mCTP), to improve mRNA stability and reduce innate immune activation.
-
Poly(A) Tail: 180 nucleotides for optimal translation.
|
| Applications |
- Assessment of mRNA delivery efficiency via fluorescence microscopy
- Quantitative luciferase reporter assays for transfection and expression analysis
|
| Concentration |
~1 µg/µL |
| Recommended Transfection Protocol |
Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
|
| Quality Control |
Validated by agarose gel electrophoresis |
| Shipping Conditions |
Shipped on dry ice |
| Storage |
Store at –80 °C |