| Cat # |
EG-301
|
| Description |
A recombinant form of the spike
glycoprotein receptor binding domain (RBD, Arg319-Phe541) from severe
acute
respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1
(GenPept: QHD43416) was produced in HEK293 cells and purified by nickel
affinity and ion exchange chromatography. This recombinant protein
contains 223 residues of the SARS-CoV-2 spike glycoprotein RBD with a
C-terminal hexa-histidine tag.
|
| Mutations
|
WT referencing Wuhan-Hu-1
|
| Tags
|
C-terminal hexa-histidine |
Purification
|
FPLC by nickel
affinity and ion exchange chromatography |
| Sequence |
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVL
YNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKI
ADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDI
STEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELL
HAPATVCGPKKSTNLVKNKCVNFGSHHHHHH |
| Mol Wt
|
25.9 kDa |
| Source
|
HEK 293 |
| Purity
|
90~95% by SDS-PAGE |
|
Applications
|
Western blot, ELISA and animal vaccination
|
| Storage Buffer |
PBS + 50% Glycerol
|
| Storage |
-20ºC ~ -80ºC |
| Shipping |
dry ice |
| References
|
|