| Description |
E coli RNA 5´ Pyrophosphohydrolase (RppH, also known as NudH/YgdP) removes pyrophosphate from the 5´ end of triphosphorylated RNA to leave a 5´ monophosphate RNA. This enzyme can split Ap5A to ADP and ATP. |
| Applications |
- RNA stability studies
- Removing a guanine cap from the 5′ end of an RNA
|
| Source |
Purified from a recombinant E. coli strain carrying the E coli RppH gene |
| Components |
- E coli RNA 5´ Pyrophosphohydrolase, 5,000 U/ml in storage buffer: 20 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.01% Triton® X-100, pH 7.5 @ 25°C
- 10X Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl2, 10 mM DTT, pH 7.9 @ 25°C
|
| Unit Definition |
One unit is the amount of enzyme that converts 1 µg 300 mer RNA transcript into a XRN-1 digestible RNA in 30 minutes at 37°C. |
| Quality Control |
- The absence of endonuclease, exonuclease, phosphatases, and RNase activities is confirmed by appropriate quality tests.
|
| Storage |
-20°C. |