| Description |
FastÄQTM Taq DNA polymerase is an engineered Taq to achieve rapid PCR without compromising specificity and accuracy. It can extend a kilobase of sequence in 15 seconds and with accuracy 50 times higher than Taq DNA Polymerasey. This ultra-thermostable polymerase can be used for amplifying sequencing libraries. |
| Source |
A recombinant E. coli strain carrying the engineered FastÄQTM Taq DNA polymerase gene. |
| Applications |
- Routine PCR amplification of up to 20 kb DNA fragment.
- Blunt-end PCR cloning.
- Sequencing library preparation
|
| Unit Definition |
One unit is defined as the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble form at 74°C in 30 minutes. |
| Features |
- High yield, high sensitivity, ultrafast FastÄQTaq DNA Polymerase
- Purity: >99%
- Specific Activity: >10,000 U/mg
- Stable at 4°C
|
| Components |
- FastÄQTM Taq DNA polymerase - 5 units/µL in 50% glycerol, 20 mM Tris-HCl, pH 7.4, 40 mM NaCl,100 mM KCl, 0.1 mM EDTA, and enzyme stabilizers.
- 10X Reaction Buffer: 160 mM (NH4)2SO4 , 670 mM Tris-HCl, pH 8.8 (at 25 °C), 15 mM MgCl2 , 0.1% Tween 20
|
| Storage |
FastÄQTM Taq DNA polymerase is stable for 2 years when stored in a constant temperature freezer at -20°C. |
| Shipping |
4°C |