| Product Name |
E1A binding protein p300, CH1 Domain |
| Size |
10,000 U |
| Description |
This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.
Human p300 and CBP (CREB binding protein) are highly related transcriptional coactivators. Both proteins have been identified through protein interaction assays (1-3). In addition to interacting with a variety of cellular factors and onco-proteins, loss of the wild type CBP alleles in isolated tumors suggests that CBP/p300 might serve as tumor suppressors (4). The ability of p300 to acetylate many transcription factors, including p53, E2F, TFIIE, and TFIIF etc. demonstrated a novel mechanism of targeted p300 regulation of gene expression (5-7). The Zn(2+)-binding cysteine/histidine-rich 1 (CH1) domain (amino acid 302-531) of p300/CBP binds many of these transcription factors
|
| Applications |
- protein-protein interaction assay
- in vitro transcription assay
- in vitro acetylation assay
- cell growth assay
|
| Source |
E. coli |
| Fusion Tag |
6X His |
| Purification Method |
FPLC |
| Unit Definition (Activity) |
1 unit equals to 1 nanogram of purified protein. 1-5 units are sufficient for a gel mobility shift assay in a 20µl reaction; 50-100 units are sufficient for reconstituted transcription assay and 100-200 units are sufficient for a protein-protein interaction assay or an acetylation assay. |
| Purity |
~ 95% as determined by SDS-PAGE |
| Gene ID |
2033 |
| Accession # |
NM_001429 |
| Gene Name |
E1A binding protein p300 |
| Gene Symbol |
EP300 |
| Gene Synonyms |
p300; KAT3B; RSTS2 |
| MW |
26.0 kDa |
| Protein Sequence |
MGQQPAPQVQ QPGLVTPVAQ GMGSGAHTAD PEKRKLIQQQ LVLLLHAHKC QRREQANGEV
RQCNLPHCRT MKNVLNHMTH CQSGKSCQVA HCASSRQIIS HWKNCTRHDC PVCLPLKNAG
DKRNQQPILT GAPVGLGNPS SLGVGQQSAP NLSTVSQIDP SSIERAYAAL GLPYQVNQMP
TQPQVQAKNQ QNQQPGQSPQ GMRPMSNMSA SPMGVNGGVG VQTPSLLSDS |
| Storage Buffer |
20 mM Tris-HCl, pH 8.0, 250 mM NaCl, 1 mM DTT, 50% Glycerol |
| Storage |
-20 to -80 °C. |
| Shipping |
4°C or dry ice |
| References |
1. Stein, RW. et al., (1990) J. Virol. 64, 4421-4427
2. Chrivia, JC. et al., (1993) Nature 365, 855-859
3. Eckner, R. et al., (1994) Genes & Dev. 8, 869-884
4. Kung, AL. et al., (2000) Genes & Dev. 14, 272-277
5. Imhof, A. et al., (1997) Curr. Biol. 7, 689-692
6. Gu, W. et al., (1997) Cell 90, 595-606
7. Martinez-Balbas, MA. et al., (2000) EMBO J. 19, 662-671 |