| Description |
Recombinant Bst DNA Polymerase (large fragment) has 5’-> 3’ polymerase activity, but lacks 5’->3’ exonuclease activity. This enzyme can be used for and rapid amplification of trace amounts of DNA templates for sequencing.
|
| Applications |
- DNA sequencing through high GC regions
- Rapid amplification of trace amounts of DNA templates
- Next Generation sequencing
|
| Source |
A recombinant E. coli strain carrying the large fragment of DNA polymerase gene from Bacillus stearothermophilus |
| Unit Definition |
One unit is defined as the amount of polymerase required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65°C. |
| Components |
- Bst DNA Polymerase: 8,000 U/ml in 10 mM Tris-HCl, 50 mM KCl, 1.0 mM Dithiothreitol, 0.1 mM EDTA, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25°C
- 10X Reaction Buffer: 200 mM Tris-HCl, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, and 1.0 % Triton X-100, pH 8.8 @ 25°C
|
| Quality Control |
The absence of endonuclease, nicking activity and exonuclease activity is confirmed by appropriate quality tests. |
| Storage Condition |
-20 °C
|