| Cat # |
EG-304
|
| Description |
A recombinant form of the full
length
spike glycoprotein D614G mutant from severe acute respiratory
syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept:
QHD43416) was produced in HEK293 cells and purified by nickel affinity
and ion exchange chromatography. This recombinant protein has been
stabilized by several mutations: removing the polybasic S1/S2
cleavage site (RRAR to A, residues 682 to 685); K986P and V987P;
adding a T4 foldon trimerization domain followed by a hexa-histidine
tag
at the C-terminus. This protein forms a trimer, which is suitable for
screening of neutralizing antibodies in vitro |
Mutations
|
D614G, (682PRAR685)->A, K986P, V987P |
Tags
|
C-terminal hexa-histidine |
Purification
|
FPLC by nickel
affinity and ion exchange chromatography |
Sequence |
1 CVNLTTRTQL PPAYTNSFTR GVYYPDKVFR SSVLHSTQDL FLPFFSNVTW
51 FHAIHVSGTN GTKRFDNPVL PFNDGVYFAS TEKSNIIRGW IFGTTLDSKT
101 QSLLIVNNAT NVVIKVCEFQ FCNDPFLGVY YHKNNKSWME SEFRVYSSAN
151 NCTFEYVSQP FLMDLEGKQG NFKNLREFVF KNIDGYFKIY SKHTPINLVR
201 DLPQGFSALE PLVDLPIGIN ITRFQTLLAL HRSYLTPGDS SSGWTAGAAA
251 YYVGYLQPRT FLLKYNENGT ITDAVDCALD PLSETKCTLK SFTVEKGIYQ
301 TSNFRVQPTE SIVRFPNITN LCPFGEVFNA TRFASVYAWN RKRISNCVAD
351 YSVLYNSASF STFKCYGVSP TKLNDLCFTN VYADSFVIRG DEVRQIAPGQ
401 TGKIADYNYK LPDDFTGCVI AWNSNNLDSK VGGNYNYLYR LFRKSNLKPF
451 ERDISTEIYQ AGSTPCNGVE GFNCYFPLQS YGFQPTNGVG YQPYRVVVLS
501 FELLHAPATV CGPKKSTNLV KNKCVNFNFN GLTGTGVLTE SNKKFLPFQQ
551 FGRDIADTTD AVRDPQTLEI LDITPCSFGG VSVITPGTNT SNQVAVLYQG
601 VNCTEVPVAI HADQLTPTWR VYSTGSNVFQ TRAGCLIGAE HVNNSYECDI
651 PIGAGICASY QTQTNSPASV ASQSIIAYTM SLGAENSVAY SNNSIAIPTN
701 FTISVTTEIL PVSMTKTSVD CTMYICGDST ECSNLLLQYG SFCTQLNRAL
751 TGIAVEQDKN TQEVFAQVKQ IYKTPPIKDF GGFNFSQILP DPSKPSKRSF
801 IEDLLFNKVT LADAGFIKQY GDCLGDIAAR DLICAQKFNG LTVLPPLLTD
851 EMIAQYTSAL LAGTITSGWT FGAGAALQIP FAMQMAYRFN GIGVTQNVLY
901 ENQKLIANQF NSAIGKIQDS LSSTASALGK LQDVVNQNAQ ALNTLVKQLS
951 SNFGAISSVL NDILSRLDPP EAEVQIDRLI TGRLQSLQTY VTQQLIRAAE
1001 IRASANLAAT KMSECVLGQS KRVDFCGKGY HLMSFPQSAP HGVVFLHVTY
1051 VPAQEKNFTT APAICHDGKA HFPREGVFVS NGTHWFVTQR NFYEPQIITT
1101 DNTFVSGNCD VVIGIVNNTV YDPLQPELDS FKEELDKYFK NHTSPDVDLG
1151 DISGINASVV NIQKEIDRLN EVAKNLNESL IDLQELGKYE QYIKWPSGRL
1201 VPRGSPGSGY IPEAPRDGQA YVRKDGEWVL LSTFLGHHHH HH
|
Mol Wt
|
137.6 kDa |
Source
|
HEK 293 |
Purity
|
90~95% by SDS-PAGE |
|
Applications
|
Screening of neutralizing antibodies,
western blot, ELISA, animal
vaccination
|
| Storage Buffer |
PBS + 50% Glycerol
|
| Storage |
-20ºC ~ -80ºC |
| Shipping |
dry ice |
References
|
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