NLS-Cas9-D10A Nickase mRNA

Cat #: EG-315

   

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Product Name NLS-Cas9-D10A Nickase mRNA
Description

NLS-Cas9-D10A Nickase mRNA encodes a Streptococcus pyogenes SF370 Cas9 protein D10A mutant with nuclear localization signal (NLS) at C terminus. Unlike the wild-type CAS9, which cleaves the double strand DNA generating two break sites based on its two active domains, the NLS-Cas9-D10A Nickase has only one active domain, thus it can only cut one single strand DNA that is complementary to the guide-RNA, producing one single strand break. This mutant mRNA favors homology-directed repair and decreases the occurrence of non-homologous end joining.

Modifications
  • 5' Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
  • Pseudo-UTP
  • 5mCTP
  • PolyA: 180 nt
Application CRISPR, Genome Enginering
Concentration ~1 µg/µl
Transfection Protocol Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
QC Agarose gel electrophoresis
Shipping Dry ice
Storage -80 oC