This modified mRNA encodes the full-length SARS-CoV-2 spike glycoprotein B.1.617.2 India origin delta variant, with furin site mutated to PRRD

• 5' Cap: Capped with ARCA (Anti Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'OMeA)pG, Cap 1)
• 5’-UTR: derived from human alpha-globin RNA with an optimized Kozak sequence
• Codon Optimization: Human
• Mutations: T19R, T95I, G142D, ∆F157+∆R158, E156G, L452R, T478K, D614G, P681R, D950N, P681R, R683A, R685A, D950N
• Nucleotide Modifications: mRNA modified with both Pseudo-UTP and 5mCTP
• 3’-UTR: comprises two sequence elements derived from the amino-terminal enhancer of split (AES) mRNA and the mitochondrial encoded 12S ribosomal RNA to confer RNA stability and high total protein expression.
• PolyA: 180 nt