| Product Name |
Hyperactive PiggyBac Transposase mRNA |
| Description |
In vitro transcribed, chemically modified mRNA encoding an engineered hyperactive variant of the PiggyBac transposase. This mRNA is DNA-free, animal-origin-free, phosphatase-treated, and optimized for high efficiency in genome engineering applications.
|
| Modifications |
- 5' capped with ARCA (Anti-Reverse Cap Analog, m7(3'-O-methyl)-G(5')ppp(5')G, Cap 0) or CleanCap AG (m7G(5')ppp(5')(2'-OMeA)pG, Cap 1)
- 5' untranslated region (UTR): human hemoglobin subunit alpha 2 (HBA2)
- 3' UTR: regulatory stability elements from human α-globin and β-globin genes
- 100% substitution with pseudouridine triphosphate (Ψ-UTP)
- 30% substitution with 5-methylcytidine triphosphate (5mCTP)
- Poly(A) tail: 180 nucleotides
- Certified animal component-free
|
| Application |
Genome engineering and transposon-based gene delivery |
| Concentration |
~1 µg/µL |
| Transfection Protocol |
Lipofectamine™ MessengerMAX™ Transfection Reagent Protocol
|
| Shipping |
Dry ice |
| Storage |
-80 °C |
|