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Thermus aquaticus Muts DNA mismatch repair protein, N-6X His-MBP-Taq-Muts

Cat #: EG-9

$395.00

Qty   
Product Name Thermostable MutS Mismatch Repair Protein (6×His-MBP-Taq-MutS Fusion)
Catalog Number [Your catalog number here]
Size 100 µg (1 mg/mL stock concentration)
Description

Recombinant Thermus aquaticus MutS protein expressed as an N-terminal 6×His-MBP fusion for enhanced solubility and purification. This thermostable mismatch-binding protein specifically recognizes and binds to various DNA mismatches with high affinity across a broad temperature range (4–70°C).

Key Features:

  • Recognizes all single base mismatches (GT, CT, AG, etc.) and 1-4 bp indels
  • Maintains activity from 0–75°C with optimal binding at 65°C
  • Exhibits thermostable ATPase activity (kcat = 0.5 min-1 at 65°C)
  • Dual-affinity tags (6×His and MBP) enable multiple purification strategies
  • >95% purity by SDS-PAGE analysis
Applications
  • Error correction in synthetic DNA and gene synthesis workflows
  • High-throughput mutation detection and SNP screening
  • Isothermal nucleic acid amplification quality control
  • DNA heteroduplex analysis and mismatch characterization
Source Recombinant expression in E. coli
Protein Characteristics
  • MW: 135.9 kDa (theoretical)
  • Extinction Coefficient: 149,430 M-1cm-1 (280 nm)
  • Fusion Tags: N-terminal 6×His and maltose-binding protein (MBP)
  • Accession: AAC43637 (T. aquaticus MutS)
Quality Control
  • >95% purity by SDS-PAGE (Coomassie staining)
  • Functional validation using standardized mismatch-binding assay
  • Endotoxin level: <0.1 EU/µg
  • Sterility tested (bacterial culture)
SDS-PAGE analysis
Buffers

Storage Buffer:
20 mM Tris-HCl (pH 8.0), 250 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol

Reaction Buffer (10×):
100 mM KCl, 50 mM Tris-HCl (pH 8.5), 10 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 2% glycerol
Storage & Handling
  • Long-term: -80°C (stable for 24 months)
  • Short-term: -20°C (stable for 6 months)
  • Shipping: Dry ice
  • Note: Avoid repeated freeze-thaw cycles
Example Protocol

For error correction in gene synthesis:

  1. Purify PCR products (QIAquick kit) and elute in 10 mM Tris-HCl (pH 7.8)
  2. Denature/reanneal (95°C for 5 min, cool at 0.1°C/s to 25°C)
  3. Add 1× reaction buffer and Taq MutS (950 nM final concentration)
  4. Incubate 10 min at room temperature
  5. Add amylose resin (NEB) and incubate 30 min with gentle mixing
  6. Collect supernatant for downstream processing
References
  1. Carr PA, et al. (2004) Protein-mediated error correction for de novo DNA synthesis. Nucleic Acids Res 32(20):e162. doi:10.1093/nar/gnh160
  2. Matzas M, et al. (2005) Correcting errors in synthetic DNA through consensus shuffling. Nucleic Acids Res 33(6):e55. doi:10.1093/nar/gni054
  3. Bui CT, et al. (2007) Rapid SNP diagnostics using asymmetric isothermal amplification. Nat Methods 4:257-262. doi:10.1038/nmeth1007