ÄQStart^TM Taq DNA polymerase

ÄQStart Taq DNA polymerase contains purified monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Activated ÄQStart Taq DNA polymerase possesses 5'→3' DNA polymerase activity and a double-strand specific 5'→3' exonuclease. The polymerase does not have 3'-exonuclease activity and is free of any contaminating endo or exonuclease activities. One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

• High yield, high sensitivity ÄQStart Taq DNA Polymerase
• Hot-start technology ensures specific and efficient primer extension
• Purity (SDS-PAGE): >99%
• Specific Activity: ~80,000 Units/mg
• E.coli DNA Contamination: Undetectable by RT-PCR

• ÄQStart Taq DNA polymerase: 5 units/µL in 50% glycerol, 20 mM Tris-HCl, 40 mM NaCl, 0.1 mM EDTA, and enzyme stabilizers.
• 10X Reaction Buffer: 160 mM (NH₄)₂SO₄ , 670 mM Tris-HCl, pH 8.8 (at 25 °C), 15 mM MgCl₂ , 0.1% Tween 20