Taq DNA Polymerase

Taq DNA Polymerase is purified from E.coli cells with a cloned pol gene from Thermus aquaticus YT1. The purified enzyme is a monomeric protein with molecular weight of 94 kDa.The enzyme catalyzes 5'->3' synthesis of DNA, has no detectable 3'->5' exonuclease (proofreading) activity and possesses low 5'->3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products.

• Thermostable – half life is more than 40 min at 95°C.
• Generates PCR products with 3’-dA overhangs.
• Purity (SDS-PAGE): >99%
• Contaminants: E.coli 16S rDNA is below detection limit by RT-PCR

• Recombinant Taq DNA polymerase: 5 units/µL in 20 mM Tris-HCl (pH 7.5), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, Stabilizer and 50% (v/v) glycerol.
• 10X Standard Taq Buffer: 100 mM Tris-HCl, 500 mM KCl, 15 mM MgCl_2, pH 8.3 @ 25°C

-20°C.