| Product Name |
3C HiFi DNA Polymerase |
| Catalog Number |
EG-1027 |
| Description |
3C HiFi DNA Polymerase, a recombinant thermostable enzyme with 3’ to 5’ proofreading activity, derived from an engineered Thermococcus-like DNA polymerase gene expressed in Escherichia coli, amplifies DNA up to 20 kb with processivity and elongation rates at least fivefold higher than Pfu DNA polymerase, optimized for high-fidelity PCR at ~72°C. |
| Applications |
- High-fidelity PCR amplification
- Blunt-end PCR cloning
|
| Source |
Recombinant Escherichia coli expressing an engineered Thermococcus-like DNA polymerase gene |
| Unit Definition |
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C under standard DNA polymerase assay conditions. |
| Components |
- 3C HiFi DNA Polymerase: 5 units/µL in 20 mM Tris-HCl (pH 8.0), 40 mM NaCl, 2 mM sodium phosphate, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol, and stabilizers
- 10X Reaction Buffer: 200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM (NH4)2SO4, 100 mM KCl, 1% Triton X-100, 1 mg/mL BSA, 20 mM MgSO4
|
| Storage |
-20°C |
| Shipping |
Dry ice |
|