T4 DNA Polymerase

T4 DNA Polymerase catalyzes the extension of a primed DNA template (in the presence of template and primer) in the 5′→ 3′ direction. This enzyme exhibits a powerful 3′→ 5′ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli), while lacking any inherent 5′→ 3′ exonuclease or strand displacement functions.

• Blunting of DNA ends: fill-in of 5'-overhangs or/and removal of 3'-overhangs
• Blunting of PCR products with 3'-dA overhangs
• Single strand deletion subcloning (Ligation-independent cloning of PCR products).
• Second strand synthesis in site-directed mutagenesis.
• Probe labeling using replacement synthesis.

• T4 DNA Polymerase: 3,000 U/ml in 100 mM KPO₄, 1.0 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 6.5 @ 25°C
• 10X Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl₂, 10 mM DTT, pH 7.9 @ 25°C

-20 °C