| Product Name |
T4 DNA Polymerase |
| Catalog Number |
EG-1058 |
| Description |
T4 DNA Polymerase, a recombinant enzyme from bacteriophage T4 expressed in Escherichia coli, catalyzes 5’ to 3’ DNA synthesis with robust 3’ to 5’ exonuclease activity but lacks 5’ to 3’ exonuclease and strand displacement activities, ideal for DNA blunting and labeling applications. |
| Applications |
- Blunting of DNA ends by fill-in of 5’ overhangs or removal of 3’ overhangs
- Blunting of PCR products with 3’-dA overhangs
- Ligation-independent cloning of PCR products via single-strand deletion subcloning
- Second-strand synthesis in site-directed mutagenesis
- Probe labeling using replacement synthesis
|
| Source |
Recombinant Escherichia coli expressing the T4 bacteriophage gene 43 |
| Unit Definition |
One unit is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard DNA polymerase assay conditions. |
| Components |
- T4 DNA Polymerase: 3,000 units/mL in 100 mM potassium phosphate (pH 6.5 at 25°C), 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol
- 10X Reaction Buffer: 500 mM NaCl, 100 mM Tris-HCl (pH 7.9 at 25°C), 100 mM MgCl2, 10 mM DTT
|
| Quality Control |
- Absence of endodeoxyribonucleases confirmed by appropriate quality tests
- Functionally validated for blunting of DNA ends
|
| Storage |
-20°C |
| Shipping |
Dry ice |