| Product Name |
Human Protein Kinase A, Catalytic Subunit Alpha (PKA Cα, cAMP-dependent protein kinase catalytic subunit alpha isoform 1) |
| Overview |
Human Protein Kinase A (PKA) catalytic subunit alpha (Cα), also known as cAMP-dependent protein kinase catalytic subunit alpha isoform 1, is a serine/threonine kinase that mediates the cellular effects of cyclic AMP (cAMP) signaling. PKA Cα is the principal catalytic subunit of the PKA holoenzyme and plays a fundamental role in regulating metabolism, gene expression, cell growth, and differentiation through phosphorylation of diverse protein substrates. Upon cAMP binding to the regulatory subunits, the catalytic subunits are released and activated, enabling phosphorylation of target proteins throughout the cell.
|
| Gene/Protein Names |
- Gene: PRKACA
- UniProt: P17612
- Other Names: PKA Cα, cAMP-dependent protein kinase catalytic subunit alpha, PKA catalytic alpha, C-alpha
|
| Molecular Weight |
~40 kDa (monomer) |
| Key Features |
- Highly purified, recombinant human PKA Cα
- Active serine/threonine kinase
- Phosphorylates a broad range of protein substrates in response to cAMP signaling
- Essential for metabolic regulation, cell signaling, and gene expression
- Widely used as a model kinase in biochemical and pharmacological research
|
| Applications |
- In vitro kinase assays
- Phosphorylation studies of target substrates
- Drug discovery and inhibitor screening
- Signal transduction research
- Structural and mechanistic studies of PKA function
|
| Source |
Recombinant protein expressed in E. coli or baculovirus-infected insect cells |
| Quality Control |
- Purity >95% by SDS-PAGE
- Confirmed kinase activity in standard phosphorylation assays
|
| Formulation |
20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5 |
| Storage |
-80 °C (long-term); avoid repeated freeze-thaw cycles |
| References |
- Kim, C., Xuong, N.H., Taylor, S.S. "Crystal structure of a complex between the catalytic and regulatory (RIα) subunits of PKA." Science. 2005; 307(5710): 690-696.
- Taskén, K., Aandahl, E.M. "Localized effects of cAMP mediated by distinct routes of protein kinase A." Physiol Rev. 2004; 84(1): 137-167.
- UniProt: P17612
|
|